Cy5 filter on compound
WebEmission filter (nm) Channel Antibody labels Compatible fluorescent proteins; Blue–488 nm: 525/50: BL1: Alexa Fluor 488 FITC: eGFP Emerald eYFP: 590/40: BL2: PE PE-Alexa Fluor 610 PE-Texas Red: EYFP mCitrine Venus: 695/40: BL3: PE-Alexa Fluor 700 PE-Cy5.5 PerCP PerCP-Cy5.5 Qdot 705 TRI-COLOR : Green–532 nm: 575/36: GL1: Alexa Fluor … WebThe correction factor (CF) for Cy5 (Cyanine-5) is CF260 = 0.02, CF280 = 0.03, CF482 = 0.009, CF565 = 0.09. Unless otherwise specified, correction factor (CF) values are typically measured in ddH 2 O or PBS. Select a compound from the list below to view correction factor (CF) data. Enter keywords into the search box to filter the list.
Cy5 filter on compound
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Cy5.5 is a near-infrared (IR) fluorescence-emitting dye (excitation/emission maximum 678/694 nm). Cy7 is a near-IR fluor that is invisible to the naked eye (excitation/emission maximum 750/776 nm). It is used in in vivo imaging applications, as well as the Cy7.5 dye. See more Cyanines, also referred to as tetramethylindo(di)-carbocyanines are a synthetic dye family belonging to the polymethine group. Although the name derives etymologically from terms for shades of See more Cyanines were first synthesized over a century ago. They were originally used, and still are, to increase the sensitivity range of photographic emulsions, i.e., to increase the range of See more The word cyanin is from the English word "cyan", which conventionally means a shade of blue-green (close to "aqua") and is derived from the Greek κυάνεος/κυανοῦς kyaneos/kyanous which means a somewhat different color: "dark blue". See more Cyanines have been classified in many ways: • Streptocyanines or open chain cyanines: R2N … See more Because they yield brighter and more stable fluorescence, cyanines can advantageously replace conventional dyes such as See more http://jinpanbio.com/archives/date/2024/04/13
WebThe Cy5 fluorophore is also the basis for the lipophilic neuronal and long-term DiD cell tracing reagents, which append alkyl tails (≥12 carbons) onto the core fluorophore. For … WebCy5 Protein Labeling Kit Fluorescent labeling of primary amino groups MSDS (PDF) Datasheet (PDF) excitation and emission spectrum of Cy5 For general laboratory use. Shipping: shipped on gel packs Storage …
WebSurprisingly, I saw that the specimen labeled with Cy5 only was seen using the Cy3 method (532nm laser and BPG1 or DGR1 GE filter that cuts a 560-580 nm band) and that it looked half as bright as ... WebThe correction factor (CF) for Cy5 (Cyanine-5) is CF 260 = 0.02, CF 280 = 0.03, CF 482 = 0.009, CF 565 = 0.09. To request additional information and datasets for Cy5 (Cyanine-5), please [email protected]. Unless otherwise specified, correction factor (CF) values are typically measured in ddH 2 O or PBS.
WebApr 6, 2024 · The Cy5-labeled ssDNA pools (62.5 nM) ... the culture medium with mAbs was harvested by centrifugation at 4000 rpm and passed through a 0.22 μM filter. The first step of purification was performed by affinity column chromatography using protein A Sepharose (Repligen, Waltham, MA, USA), eluted with citrate buffer (pH 3.0), and neutralized with ...
Web49022 - ET - Cy5.5. All transmission and blocking (OD) data are actual, measured spectra of representative production lots. Spectra varies slightly from lot to lot. Optical density … dr gold\u0027s ear therapy for dogs reviewsentergy thermostat program mississippiWebApr 5, 2011 · The FACS machine at our core facility has a 670 LP (long pass) filter to detect PerCP and PerCP-Cy5.5. Yet, unlike the band pass (BP) filters these seem to accept a large chunk of the emission ... entergy verification deptWebBD Pharmingen™ PE-Cy5 (PE-Cy5) (Ex Max 496 nm and 566 nm/Em Max 670 nm) is a tandem conjugate that combines phycoerythrin and a cyanine dye. PE designed to be excited by the blue (488 nm), green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 670/20 nm bandpass filter). dr gold\u0027s ear therapy for dogs \u0026 catsWebThe molar extinction coefficient (ε) for Cy5.5 (Cyanine-5.5) is 250000 cm-1M-1 . Unless otherwise specified, molar extinction coefficient (ε) values are typically measured in ddH 2 O or PBS. Select a compound from the list below to view molar extinction coefficient (ε) data. Enter keywords into the search box to filter the list. dr gold\u0027s itch reliefBecause they yield brighter and more stable fluorescence, cyanines can advantageously replace conventional dyes such as fluorescein and rhodamines. • Cy3 and Cy5 are the most popular, used typically combined for 2 colors detection. Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while … entergy webmailWeb89401 - ET - DAPI/FITC/TRITC/CY5 Quad. Price: $1,350.00. A version of the 89000 Quad set with a quad band excitation filter, dichroic and emission filter. Useful for simultaneous live viewing of multiple channels or image-splitting applications. May also be paired with individual emission filters from 89000 set for highest SNR image-splitting ... dr.gold waidhofen an der thaya