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Easytaq酶说明书

Web合理选择耐热DNA聚合酶是PCR成败与否的一个关键因素。. 选择最合适的耐热聚合酶，是进行PCR实验首先要考虑的问题。. 许多耐热DNA聚合酶的主要区别在于 特异性、保真性、耐热性、扩增速率、扩增片段长度 等几个指标。. 耐高温的DNA聚合酶分为三类。. 第一类 ... EasyTaq® DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity.

EasyTaq DNA Polymerase

WebJan 28, 2024 · The eamB operon was amplified by PCR from E. coli genomic DNA with the primers eamB-F and eamB-R. PCR reactions were performed in a 50 μl volume containing 5 μl 10× EasyTaq buffer, 0.2 μM of each primer, 200 μM dNTPs, 0.8 mM of MnCl 2, 6 mM of MgSO 4, 50 ng of template DNA, and 1 μl EasyTaq DNA polymerase (TransGen … WebEasyTaq® DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous … trad the split https://carolgrassidesign.com

2×EasyTaq® PCR SuperMix (+dye )

WebTransScript ® Uni All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal) TransScript ® II Two-Step RT-PCR SuperMix. TransScript ® Uni Reverse Transcriptase [M-MLV, RNase H-] (High Temperature RT) TransScript ® IV One-Step gDNA Removal and cDNA Synthesis SuperMix. WebSpecification：. 1.2 ml (with Mg2+) 1.2 ml (for PAGE) Quantity：. Manual MSDS COA. Inquire Now Buy Now Add to Cart Free Sample Avaliable. Product Details. 10× EasyTaq ® Buffer provides the preferred pH and ionic strength for PCR amplification reactions performed with EasyTaq ® DNA Polymerase. http://www.coolaber.com/usr/2160/File/F/F3299.pdf trad the night we met

TransTaq®-T DNA Polymerase

Category:EasyTaq® DNA Polymerase - TransGen Biotech

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Easytaq酶说明书

Directed evolution of an EamB transporter for improved L-cysteine ...

WebSpecificity. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. It lacks 3'-5' exonuclease activity. EasyTaq® DNA Polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE. WebEasyTaq®. Specificity. Extension rate is about 1-2 kb/min. Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be cloned into pEASY®-T vectors. Amplification of genomic DNA fragment up to 4 kb. Characteristics. EasyTaq® PCR SuperMix is a ready-to-use mixture of EasyTaq® DNA Polymerase, dNTPs and ...

Easytaq酶说明书

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WebEasyTaq® PCR SuperMix is a ready-to-use mixture of EasyTaq® DNA Polymerase, dNTPs and optimized buffer. The SuperMix is provided at 2xconcentration and used at 1x … Web相对分子质量为94的Taq DNA聚合酶的活性较高，大约为200000 U/mg，在合成DNA时有一个较高的最适温度75～80℃。Taq DNA聚合酶虽然在90℃以上合成DNA的能力有限，但高温时仍比较稳定。有实验证明，在92.5℃、95℃和97.5℃时，PCR混合物中的Taq DNA聚合酶分别经130 min、40 min和5～6 min后，仍可保持50%左右的活性 ...

Web地址：北京市海淀区永泰庄北路1号中关村东升国际科学园4号楼. E-mail：[email protected]. 客服热线：+86-400 898 0321 WebMay 14, 2013 · Ex-Taq酶 说明. TaKaRaExTaqTaKaRaCode：DRR001A包装量：250制品说明本制品是应用LAPCR原理研制的具有3′5′Exonuclease活性（Proofreading活性）的耐 …

WebMay 19, 2016 · Fidelity is 18 times higher than EasyTaq® DNA Polymerase. The specificity is higher than antibody based or chemically modified hot start DNA polymerases. Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors. WebTaKaRa Ex Taq®. 使 用 说 明 书. TaKaRa Code ：DRR001A. 包装量：250 U 制品说明. 本制品是应用 LA PCR 原理研制的具有 3′→5′ Exonuclease 活性（Proof reading 活性）的 …

WebApr 8, 2024 · Briefly, 1ul of cDNA was added to the mix comprising of 10X EasyTaq buffer (2ul), It is made available under a CC-BY-NC-ND 4.0 International license. (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.

WebMar 25, 2024 · • Each batch of EasyTaq® DNA Polymerase has been assayed for amplification efficiency of the p53 gene from 10 ng of human genomic DNA. Storage Buffer 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers 10×EasyTaq® Buffer (with Mg2+) 200 mM Tris-HCl (pH 8.3), 200 mM KCl, 100 mM (NH … trad themWebProduct Details. EasyTaq® PCR SuperMix is a ready-to-use mixture of EasyTaq® DNA Polymerase, dNTPs, and optimized reaction buffer at a concentration of 2×. For DNA amplification, simply add the template, … trad thirdWebCitations & References. PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Simply add template and primers, reducing set-up time by half. PCR SuperMix contains Mg 2+, dNTPs, and recombinant Taq DNA Polymerase at concentrations sufficient for routine PCR of fragments up to 5 kb. trad thotsWebEasyTaq ® D NA Polymerase是从克隆有Thermu aquaticus DNA Polymerase基因的载体，在大肠杆菌中经诱导表达、分离纯化而成，其分子量为94 kDa。 EasyTaq ® DNA … the san miguel mine montanaWebMay 29, 2014 · Easy-Taq 使用说明书使用,说明,中文,使用说明书,说明书,Easy,easy,中文使用 trad thoseWebMay 4, 2011 · Ex Taq酶与普通的Taq酶有什么区别. #热议# 哪些癌症可能会遗传给下一代？. Ex Taq酶的活性维持更好，相对于普通Taq可以有效扩增更长的片段。. 那Ex Taq酶是否 … the sanny side up mindset planner 2.0WebDec 7, 2012 · TaKaRa LA Taq说明书.pdf. TaKaRaLATaqTakaraCode量：125Units制品说明本制品是应用LAPCR原理研制的具有3活性（Proofreading活性）的耐热性DNA聚合酶。. 无论是扩增短链DNA还是长链DNA，其效率都优于其它同类产品，尤其在扩增大于10kb的DNA片段方面，其优势更加明显，而且保真 ... the sanno