Echinocandin b deacylase
WebObjective:To construct genetic engineering strain containing more copies of echinocandin B deacylase by ΦC31-directed site-specific recombination.Methods:The gene integrated plasmid pYGCQ-03-13 containing echinocandin B deacylase gene was constructed and transformed into Actinoplanes utahensis strain SIPI-T2001 with conjugation.The … WebSimilarly, echinocandin B deacylase of Actinoplanes utahensis was used in the second-type synthesis of the ECB antifungal agent. The case studies focus on two types of enzyme applications for pharmaceutical development. Demethylmacrocin O-methyltransferase, macrocin O-methyltransfera
Echinocandin b deacylase
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WebSep 8, 2024 · Efficient bioconversion of echinocandin B to its nucleus by overexpression of deacylase genes in different host strains. Shao L, Li J, Liu A, Chang Q, Lin H, Chen D. Appl Environ Microbiol, 79(4):1126-1133, 07 Dec 2012 Cited by: 6 articles PMID: 23220968 PMCID: PMC3568618. Free to read WebEchinocandin B deacylase, a cell-associated enzyme from Actinoplanes utahensis , is purified to near homogeneity in a process comprising hydrophobic interaction chromatography, cation-exchange...
WebIt could be demonstrated that the echinocandin B deacylase was independent of cofactors, metal ions and reducing agents. The substrate specificity was rather broad and the echinocandin B deacylase also accepted 6-membered and more complex cyclic peptides [ … WebEchinocandin B deacylase (ECBD) from Actinoplanes utahensis can be applied to produce echinocandin B nucleus (ECBN), an essential intermediate of the echinocandins …
WebAntibacterial lipopeptides and methods for their preparation and use转让专利转让专利 WebEchinocandin B is a cyclic hexapeptide echinocandin antibiotic isolated from Aspergillus nidulans var. echinulatus with specific anti-yeast activity. ... L, Li J, Liu A, Chang Q, Lin H, Chen D. Efficient bioconversion of echinocandin B to its nucleus by overexpression of deacylase genes in different host strains. Appl Environ Microbiol. 2013 ...
WebSpecies: Actinoplanes utahensis Subunit composition of echinocandin B deacylase = [ECB subunit 2][ECB subunit 1] Summary: The enzymatic activity to deacylate …
WebJan 10, 2024 · echinocandin B deacylase Keywords Streptomyces Heterologous promoter 5′-UTR RBS σhrdB 1. Introduction Streptomyces is characterized by its diverse secondary metabolism and is the primary natural origin of clinically useful antibiotics ( Chan et al., 2012; Nett et al., 2010). moe mathematics syllabusWebMar 1, 2000 · The deacylase was salt-solubilized, heat-treated and purified to apparent homogeneity by a 3-step chromatographic procedure. The enzyme was a heterodimer … moema wertheimer cnpjWebMay 18, 1995 · Echinocandin B deacylase is purified to near homogeneity from Actinoplanes utahensis by a process comprising, in order, extracting the soluble enzyme, … moe mathews richmond vaWebOct 23, 2024 · Deacylation of Echinocandin B to Echinocandin B nucleus is catalysed by the acylase (also named as deacylase). This enzyme is currently identified from Actinoplanes utahensis NRRL 12052. It is a membrane-associated heterodimer composed of 63-kDa and 18- to-20-kDa subunits, and the expression of its activity is not affected by … moe mitch sweatpantsWebMar 1, 2000 · produces echinocandin B, a neutral lipopeptide. A deacylase from Actinoplanes utahensis catalyzes cleavage of the linoleoyl group from echinocandin B, a key step in generating a potential antifungal agent. Virtually all (99.8%) deacylase activity was cell-associated. The deacylase was salt-solubilized, heat-treated and purified to … moe meaning accountingWebEchinocandin B deacylase is purified to near homogeneity from Actinoplanes utahensis by a process comprising, in order, extracting the soluble enzyme, heating to 60° C., … moe moes bulldog rescue of the rockiesWebMetaCyc Pathway: echinocandin B degradation: Detail Level: This view shows enzymes only for those organisms listed below, in the list of taxa known to possess the pathway. If an enzyme name is shown in bold, there is experimental evidence … moe meaning in aviation