WebDec 10, 2024 · The overall morbidity of LSD was 4.48% among 30 dairy farms. Skin nodular biopsy, whole blood and serum samples (n= 66) were collected for the diagnosis of LSD by histopathology, PCR and sequencing. The envelope protein gene (P32), Fusion protein (F) and DNA dependent RNA polymerase 30 kDa subunit (RPO30) genes were targeted for … WebApr 22, 2024 · LSD- infected cattle were identified by PCR with previously published primers (Ireland and Binepal 1998 ). The total volume of the PCR reactions was 25 μl, comprising 12.5 μl of 2X Taq PCR master mix (Himedia, India), 5.0 μl of DNA template, 1.0 μl of each primer (10 pmol), and 5.5 μl of nuclease free water.
A capripoxvirus detection PCR and antibody ELISA based on the …
WebMar 13, 2024 · Using routine laboratory tests Capripoxviruses cannot be distinguished (Kitching, 1986; Davies and Otema, 1981). Polymerase chain reaction (PCR) offers a rapid and sensitive diagnostic technique for capripoxvirus genome detection (Ireland and Binepal, 1998; Heine et al., 1999). Webimmunoassays ( Ireland and Binepal, 1998). In Egypt, LSDV was first isolated and identified from cattle during two outbreaks in Suez and Ismalia governorates on 1989 ( House et al., … hilton palm dubai map
Detection of Capripoxvirus DNA Using a Field‐Ready Nucleic Acid ...
http://www.uni-sz.bg/bjvm/BJVM-September%202413%20p.159-169.pdf WebApr 6, 2005 · ing, Hammond & Chand 1994; Carn 1995; Ireland & Binepal 1998; Heine, Stevens, Foord & Boyle 1999). The disease is of economic importance because of … Webcapripoxvirus virus (Ireland & Binepal 1998, Mangana-Vougiouka 1999, Hein et al 1999). In this study a PCR assay based on capripoxvirus P32 gene was developed to identify capripoxvirus DNA in cell culture and biopsy samples by detecting 390 bp band in agar gel electrophoresis. MATERIALS AND METHODS Viruses and cells. 1. The capripoxvirus strains ez teak flooring