Webcompatible with both TA cloning and blunt-end cloning, and increases the positive rate of cloning. Storage Store at -30 ~ -15°C and transport at ≤0°C. Applications It is applicable for the ligation of blunt-end or A-tailed PCR products. Notes For research use only. Not for use in diagnostic procedures. C603 Ultra-Universal TOPO Cloning Kit ... WebQuick and efficient PCR cloning with TOPO TA cloning. Clone PCR-amplified DNA fragments (blunt or A-overhang) directly into a choice of over 40 subcloning, sequencing, or …
8/GW/TOPO TA Cloning Kit - Thermo Fisher Scientific
WebTOPO TA Cloningfi Kits are shipped on dry ice. Each kit contains a box with TOPO TA Cloningfi reagents (Box 1) and a box with One Shotfi Chemically Competent or ElectrocompŽ cells (Box 2). Store Box 1 at -20°C and Box 2 at -80°C. Types of TOPO TA Cloningfi Kits TOPO TA Cloningfi Kits are available with either pCRfi2.1-TOPOfi or ... WebUltra-Universal TOPO Cloning Kit is the fourth-generation TOPO cloning kit. The kit uses upgraded Topoisomerase with the optimal buffer and has higher enzyme activity. The addition of the second-generation blunting factor and vector self-ligation inhibitor makes this product compatible with both TA cloning and blunt-end cloning, and increases ... how to save stardew
Problem with topo cloning strategy. ResearchGate
Web3 rows · Features of TOPO™ TA Cloning™ Kits for Sequencing: • Get more sequence— allows for more ... The TOPO™ TA Cloning™ Kits for Sequencing provide a highly efficient, 5-minute… TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes WebCloning. The TOPO TA Cloning Kit from Invitrogen was used to clone the lysin B gene. The instructions provided were followed without modifications. Results The central goal of this research was to insert the lysin B gene in LestyG into an inducible promoter, and then insert this into E. coli for storage and future research. WebTOPO ® TA Cloning ® Kits are available with pCR ™ 2.1-TOPO ® or pCR ™ II-TOPO ® vector. Kits with competent cells are available with One Shot ® Chemically or … north face women zippered fleece hoodie