Web过氧化氢酶(CAT)是一种酶类清除剂,又称为触酶,是以铁卟啉为辅基的结合酶.它可促使H2O2分解为分子氧和水,清除体内的过氧化氢,从而使细胞免于遭受H2O2的毒害,是生物防御体系的关键酶之一.CAT作用于过氧化氢的机理实质上是H2O2的歧化,必须有两个H2O2先后与CAT相遇且碰撞在活性中心上,才能发生反应.H2O2浓度越高,分解速度越快. 几乎所有的 … WebSOD、MDA、GSH f改良荧光法测定还原型谷胱甘肽 原理:OPT 在 PH 为 8.0 的时候与还原型谷胱甘肽合成 GSH-OPT,在一定的激发波长和发射波 长下进行荧光测定,从而对 GSH 进行定量。 仪器:荧光分光光度计 试剂:1)0.1%邻苯二甲醛(OPT)溶液:临用前称取 OPT10mg 溶于 10ml 甲醇,混匀。 2)0.1mol/L 的磷酸盐缓冲液(含 5mmol/L EDTA) …
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Web16 uur geleden · Introductionβ-Mannanase is a plant cell wall remodeling enzyme involved in the breakdown of hemicellulose and plays an important role in growth by hydrolyzing the mannan-like polysaccharide, but its function in adaptation to salt stress has been less studied.MethodsBased on cloned the mannanase (MAN) gene from Mirabilis jalapa L., … Web17 feb. 2024 · To further investigate the effect of curcumin, the activities of 8-OHdG, MDA, CAT, SOD, and GSH-Px were measured by using ELISA assays. As shown in Fig. 1b–f, the activities of 8-OHdG and MDA significantly increased in the model group and the curcumin group compared with those in control group (P < 0.05). many hands trading
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Web30 aug. 2024 · The serum CAT, GSH, SOD and MDA levels differed significantly … Web1 nov. 2024 · The absorbances recorded for the SOD, GSH, MDA, and CAT assays were 450 nm, 340 nm, 532 nm, and 520 nm, respectively, using a microplate reader (Spark ® 20M Multimode Microplate Reader, Tecan, Switzerland). All assays were … WebCvetkovic et al. assessed the cytological and laboratory alterations in contralateral gonad after twist in a trial on 28 grown-up male Wistar rodents, and the authors identified the alterations in... kpsc group a posts