Web24 Mar 2024 · On day two, levels of TFEB (S211A)-GFP and TFEB targets were down to Dark levels, however the AT8 + and AT180 + p-Tau levels seem to have gradually raised but still remained significantly lower than their starting levels . Taken together, for the first time, these results suggest that light-induced, optogenetic-based expression of TFEB can … Web11 Dec 2024 · Previous studies showed TFEB is a nucleocytoplasmic shuttling protein and phosphorylated TFEB is captured by YWHA thus remains in the cytosol. Given that TFEB could translocate into the nucleus under numerous of cellular stress, we wondered whether oxidative stress induced by ROS could affect nucleocytoplasmic shuttling of TFEB.
PTEN Deficiency Facilitates Exosome Secretion and Metastasis in ...
WebTFEB is a transcription factor that regulates both autophagy and lysosomal biogenesis via rapid translocation to the nucleus from the cytosol and lysosomes upon starvation (4, 12– 14). Correspondingly, overexpression of TFEB has been shown to affect the expression of a unique set of genes that are related to lysosome function and autophagy (5). Web2 Feb 2024 · To test whether TFEB or TFE3 can induce GPNMB expression, we expressed constitutively nuclear mutants of TFEB (TFEB-S211A) or TFE3 (TFE3-S321A) in T47D and … smith \u0026 burgess llc
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Web17 Nov 2024 · TFEB and TFE3 also regulate expression of genes implicated in many other cellular pathways, including autophagy, immune response, mitochondrial biogenesis, … Web5 Jan 2024 · Specifically, TFEB S211A-GFP, unlike wild-type TFEB-GFP, diffusely localized throughout the cytosol and nucleus (Fig. 1E, F). Figure 1. Torin1 enhances TFEB S211A … Web11 May 2012 · S211 is a novel MTORC1-dependent phosphorylation site in TFEB required for the binding to YWHA. (A) Coomassie blue stained SDS-PAGE gel of co-immunopreciptated TFEB and YWHA from HeLa (CF7) cells treated with either DMSO or PP242. The asterisk (*) indicates IgG heavy and light chains. The arrowhead denotes actin. riverfest amphitheater