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Tfeb-s211a

Web24 Mar 2024 · On day two, levels of TFEB (S211A)-GFP and TFEB targets were down to Dark levels, however the AT8 + and AT180 + p-Tau levels seem to have gradually raised but still remained significantly lower than their starting levels . Taken together, for the first time, these results suggest that light-induced, optogenetic-based expression of TFEB can … Web11 Dec 2024 · Previous studies showed TFEB is a nucleocytoplasmic shuttling protein and phosphorylated TFEB is captured by YWHA thus remains in the cytosol. Given that TFEB could translocate into the nucleus under numerous of cellular stress, we wondered whether oxidative stress induced by ROS could affect nucleocytoplasmic shuttling of TFEB.

PTEN Deficiency Facilitates Exosome Secretion and Metastasis in ...

WebTFEB is a transcription factor that regulates both autophagy and lysosomal biogenesis via rapid translocation to the nucleus from the cytosol and lysosomes upon starvation (4, 12– 14). Correspondingly, overexpression of TFEB has been shown to affect the expression of a unique set of genes that are related to lysosome function and autophagy (5). Web2 Feb 2024 · To test whether TFEB or TFE3 can induce GPNMB expression, we expressed constitutively nuclear mutants of TFEB (TFEB-S211A) or TFE3 (TFE3-S321A) in T47D and … smith \u0026 burgess llc https://carolgrassidesign.com

Transcription factor EB coordinates environmental cues to ... - PNAS

Web17 Nov 2024 · TFEB and TFE3 also regulate expression of genes implicated in many other cellular pathways, including autophagy, immune response, mitochondrial biogenesis, … Web5 Jan 2024 · Specifically, TFEB S211A-GFP, unlike wild-type TFEB-GFP, diffusely localized throughout the cytosol and nucleus (Fig. 1E, F). Figure 1. Torin1 enhances TFEB S211A … Web11 May 2012 · S211 is a novel MTORC1-dependent phosphorylation site in TFEB required for the binding to YWHA. (A) Coomassie blue stained SDS-PAGE gel of co-immunopreciptated TFEB and YWHA from HeLa (CF7) cells treated with either DMSO or PP242. The asterisk (*) indicates IgG heavy and light chains. The arrowhead denotes actin. riverfest amphitheater

Up-regulation of lysosomal TRPML1 channels is essential for

Category:Optical induction of autophagy via Transcription factor EB (TFEB ...

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Tfeb-s211a

TFEB and TFE3 are novel components of the integrated stress …

WebFor mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb. Depositing Lab. Reuben Shaw. Publication. Young et al Genes Dev. 2016 Mar … Web17 Aug 2024 · In particular, TFEB S142A showed impaired phosphorylation of S142 and S138; TFEB S138A showed impaired phosphorylation of S138, but normal S142 phosphorylation; importantly, in TFEB S211A, which still retained the ability to be exported from the nucleus, S142 and S138 were normally phosphorylated (Fig. 4c). These data …

Tfeb-s211a

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WebTFEB acts as a link between autophagic process and lysosome biology [160], interacting with mTORC1 complex but not with mTORC2 controlling the lysosomal localization and function of mTORC1 [161].... Web15 Nov 2013 · Importantly, our analyses established that the induction of TFEB-S211A markedly impaired the proliferation and tumorigenic potential of Eµ-Myc lymphoma. Mechanistically, this proliferative arrest was associated with the induction of the senescence program, and with an increased extracellular acidification rate.

Web11 Feb 2013 · At stationary state, TFEB-S211A accumulates in the nucleus but also displays stable association with lysosomes ( Fig. 1, B and D; Martina et al., 2012 ). As expected, depletion of raptor did not affect the ability of TFEB-S211A to associate with lysosomal membranes ( Fig. 1, B and D ).

WebThe master regulator of lysosome biogenesis, TFEB, is regulated by MTORC1 through phosphorylation at S211, and a S211A mutation increases nuclear localization. However, … Web11 Feb 2013 · The increased ability of the TFEB-S211A mutant to associate with lysosomes when compared with TFEB-WT suggests that the binding of 14-3-3 to TFEB may mask …

Web5 Jan 2024 · Specifically, TFEB S211A-GFP, unlike wild-type TFEB-GFP, diffusely localized throughout the cytosol and nucleus (Fig. 1E, F). Figure 1. Torin1 enhances TFEB S211A nuclear localization. (A) Validation of the newly generated p-TFEB (S211) antibody. HeLa cells were transfected with the indicated MYC-tagged plasmids (WT, wild-type TFEB; …

Web25 Jan 2016 · TFEB and TFE3 are novel components of the integrated stress response. José A Martina Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA. Author profile Search articles by ORCID 0000-0001-5814-8807 Martina JA1, Heba I Diab smith\u0026burtonWeb4 Mar 2024 · The master regulator of lysosome biogenesis, TFEB, is regulated by MTORC1 through phosphorylation at S211, and a S211A mutation increases nuclear localization. … riverfest balloon lightingWeb12 Apr 2024 · PLX4720 ablated TFEB S142 phosphorylation in A375 cells (Fig. 3a ). An in vitro kinase assay using bacterially purified proteins showed that active ERK directly … riverfest auto showWebTFEB-mediated endocytosis drives assembly of the MTORC1-containing nutrient sensing complex through the formation of endosomes that carry the associated proteins RRAGD, the amino acid transporter SLC38A9, and activate AKT/protein kinase B (AKT p-T308). smith \u0026 burgess fabricationWebNational Center for Biotechnology Information smith \u0026 burns llpWeb28 Jul 2024 · Flag-tagged S142A/S211A-TFEB mutant was cotransfected, in HEK293 cells, with a GFP-tagged TFEB construct (wild-type, S142A/S211A, or S142D/S211D). Co-immunoprecipitation analysis, using Flag antibody, … smith \u0026 byford gasWeb6 Dec 2024 · However, expression of TFEB-S211A and TFE3-S321A led to a near uniform pattern of p53 expression, predominantly localized in the nuclei of HeLa cells (Figure 5C). Given our data in RAW264.7 cells suggesting that loss of TFE3 and TFEB decreases p53 half-life, we performed a complimentary cycloheximide chase experiment to test if … smith\u0026buckner funeral home nc