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Topflash/fopflash reporter

Web14. máj 2024 · TOPFlash/FOPFlash Luciferase Reporter Assays. Wnt-specific TOPFlash/FOPFlash luciferase reporter assays were used to evaluate the effects of these compounds on β-catenin–dependent transcription. WebArrowed cells showed nuclear staining of β-catenin. ( G ) Huh7 cells were transfected with equal amounts of either Super8xTOPFlash reporter or Super8xFOPFlash reporter constructs. After 48 hours, the TopFlash/FopFlash luciferase activity of CD133 + and CD133 - Huh7 cells was measured. Values shown are relative luminescence.

Topflash Upstate Biotechnology Inc Bioz

WebI did lipofectamine transfection in 12-well plates with 0.5M of cells and 0.8ug of TOPFLASH plasmid in each well. After 24h, I changed to media to serum-free media supplemented … WebTOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF … fantoomkwarts https://carolgrassidesign.com

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WebTOP or FOP plasmid + Opti-MEM and Lipofectamine + Opti-MEM are incubated at room temp for 5 min respectively. Both solutions are then mixed and incubated for another 15 min. Then, the mixture is... WebThis is a CONTROL luciferase reporter with the TCF/LEF sites of Super8XTOPflash (construct M50, Addgene Plasmid #12456) being mutated. There are 6 mutated TCF/LEF … WebB, TC1 knockdown effect on TOPFLASH analysis using HEK293 cells. The Cby-induced suppression of the reporter activity was augmented as TC1-siRNA was increased to 10 … fantoons

Fopflash Luciferase Reporter Plasmid Upstate Biotechnology Inc …

Category:FOPFlash(报告基因质粒)(D2503-1μg) - beyotime.com

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Topflash/fopflash reporter

Fopflash Addgene inc Bioz

Web4. apr 2024 · Biological and molecular characteristics of GC cell lines and PDOs upon PMCA4 inhibition were investigated using RT-qPCR, western blot, and TOPflash/FOPflash reporter assay. WebTOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF …

Topflash/fopflash reporter

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Web2. apr 2024 · Figure 6.Over-expression ofPRRX2 enhanced the activity of TOPflash/FOPflash dual-luciferase reporter gene in the SGC-7901 cells.Mean±SD.n=3.*P0.05vspFlag-NC. WebThe FOPflash luciferase reporter plasmid containing mutated TCF/LEF binding sites (mTCF) served as a negative control. Techniques Used: Luciferase, Activity Assay, Cotransfection, …

WebReporter Vector primer 4 (RVprimer4) binding region: 3746-3765: ColE1-derived plasmid replication origin: 4003: Ampicillin: 4794-5654: Synthetic poly(A) signal/transcriptional pause site: 5759-5912: Reporter Vector primer 3 (RVprimer3) binding region: 5861-5880 WebAddgene inc topflash fopflash luciferase reporter plasmids Topflash Fopflash Luciferase Reporter Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

Web26. dec 2024 · topflash is a tcf reporter plasmid containing two sets of three copies of wild-type tcf binding sites driven by the thymidine kinase minimal prompter and upstream of a … Web18. jún 2014 · The TOPFlash luciferase reporter gene, which contains 3 copies of a TCF/LEF binding site, and FOPFlash luciferase reporter, identical to TOPFlash except the TCF/LEF sites have been mutated, were purchased from Addgene (Cambridge, MA, USA) [19]. The pRL renilla luciferase vector was from Promega (Madison, WI, USA).

Web21-169 Sigma-Aldrich FOPflash (mutant TCF binding sites) Transfection grade T cell factor (TCF) reporter plasmid containing 2 full & one incomplete copy of the Tcf binding site (mutated) followed by 3 copies in the reverse orientation. Serves as a negat More>> fantop albumyWebTOPflash (TCF-Reporterplasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase minimal promoter and Luciferase open reading frame.; find Sigma-Aldrich-21170 MSDS, related peer-reviewed papers, … corona teststation mühlheim am mainWeb14. aug 2015 · zt200 TOPFlash 载体 Millipore 信号通路报告载体系统: zt201 FOPFlash 载体 ... zt1223 pGL3-FOXO-reporter-luci . zt1224 pGL4.13 . zt1225 pGL4.18 . zt1226 pGL4.19 . zt1227 pGL4.74 . zt1228 pGL5luci . zt1229 pGreen-puro . zt1230 pGZ . zt1231 pHAGE-CMV-dsRed-UBC-GFP-W ... corona teststation kreis stormarnWebLentiviral-TOP-dGFP-reporter (Plasmid #14715) Print Enlarge View all sequences Purpose Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF … corona-teststation-lechhausenTOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase minimal promoter and Luciferase open reading frame. fantoosh sketchingWebDescription. FOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed by three … corona teststation lingenfeldWebFOPflash (mutant TCF binding sites) Transfection grade T cell factor (TCF) reporter plasmid containing 2 full & one incomplete copy of the Tcf binding site (mutated) followed by 3 … fantoosh sentence